Speaker Biography

Rupam Tripathi

UCMS,College of Dental Surgery,Nepal

Title: In-vitro comparison of dentinal tubule disinfection with aloe-vera extract, 2% chlorhexidine gel and calcium hydroxide

Rupam Tripathi
Biography:

Dr Rupam Tripathi is Lecturer in UCMS,College of Dental Surgery,Nepal since 2016. She did BDS and MDS in Conservative Dentistry and Endodontics in UCMS,college of Dentistry, Nepal. She had done  Poster and paper presentation in  the several conferences of Nepal .She had attended IFEA,2018 which was held in seoul and presented poster over there.She is a member of Conservative dentistry and Endodontics of Nepal.

 

Abstract:

Introduction

Intracanal medicaments are used as an antibacterial agent to eliminate residual bacteria in a root canal after instrumentation and irrigation, to render any remaining canal content inert, to dissolve tissue and to control persistent seepage of apical fluids into the root canal system. This study aims to compare the antimicrobial efficacy of 2% chlorhexidine gel, calcium hydroxide and aloe-vera extract as an intracanal medicament against E.faecalis.

Methods

Sixty extracted single-rooted human teeth were prepared with standard method. After contaminating the canals with E.Faecalis and incubated for seven days, the samples were divided into four groups (n=15). Normal saline was used as control group. The teeth in each group were treated with normal saline, calcium hydroxide, 2% chlorhexidine gel and aloe-vera extract. Microbial samples were obtained from the dentinal shavings of root and colony forming units (CFU) of E.faecalis were recorded after 24 hrs, 72 hrs and 7 days.

Result

Mean CFU were lowest for Aloevera extract and chlorhexidine gel in 24 hrs whereas it increases in 48 hrs and 72 hrs for Aloevera extract. Mean CFU of calcium hydroxide was found to increase at 24 hrs and decreases at 72 hrs and 7 days.

Conclusion

Antimicrobial efficacy of aloe-vera extract was found to be comparable to 2% chlorhexidine gel only for shorter duration as mean CFU were lowest for both medicaments in 24hrs . Mean CFU of calcium hydroxide was found to increase at 24hrs and decreases at 72hrs and 7 days.