25th Euro Dentistry Congress

Biography:

Yasemin Kulak Ozkan  has completed her graduate education in Marmara University, Faculty of Dentisty in 1987. She became a Research Assistant in  the Faculty of Dentistry Department of Prosthodontics in the same year. She became an Associate profesor in 1996 and became Professor in 2001. She became a Vice Dean in 2004-2007.  She was elected president of TPID (Turkish Prostodontics and Implantology Association) at the 2016 and since 2014 she has  been running the dean of University  of Marmara Faculty of Dentistry  and the Head  of the department of prosthesis,Istanbul, TURKEY. She has various  published more than 60  papers in reputed journals and has been serving as an editorial board member of repute.        

Abstract:

The aims of this clinical study were to compare the marginal and internal adaptation of all-ceramic crowns generated with CAD/CAM before cementation and examine the clinical outcomes at baseline and 6th  month after luting. Materials and methods: A total of 45 crowns were placed in 38 patients, including 15 lithium disilicate ceramic crowns (LDS, IPS e.max CAD Blocks, Ivoclar, Vivadent, Schaan, Liechtenstein), 30 lithium disilicate stengthened lithium aluminosilicate glass-ceramic crowns (LAS, n!ce® CAD Blocks, Institut Straumann AG, Basel, Switzerland). The marginal and internal gaps of crowns were recorded by using a replica technique. The replica specimens were sectioned bucco-lingually and mesio-distally and the thickness of silicone layers was examined by computerized light microscope at × 40 magnification. Twenty reference points per tooth were measured, and mean marginal and internal gaps were recorded. Restorations were clinically assessed at baseline and 6 months after cementation by using modified USPHS criteria, plaque and gingival indexes and patients satisfaction criteria. Data was analysed by using “Mann-Whitney U” and “Wilcoxon Signed Rank” tests (P<0.05). Results: After 6 month-observation period, total survival rates of LDS and LAS groups were 100%. There were no clinically identified cases of crown fracture or surface chipping. The mean marginal gaps were 53. 2963 (± 11. 2691) μm for LDS group and 51. 6703 (± 11. 2381) μm for LAS group. The highest gap value was observed at the occlusal area and lowest one at the marginal area (P<0,05). There was no significant difference in relation to material difference. Conclusions: Regarding all of the clinical evaluation criteria all 45 crowns exhibited clinically acceptable scores within an average evaluation time of 6 months. Early results indicate that LAS crowns may be an effective option for all-ceramic restorations.
 

Biography:

Dirk Neefs  graduated as a dentist in 1987 at Brussels University, specialized in oral rehabilitations with dental implants at Liege University in 1992. He has his dental Office in Antwerp Belgium and Oviedo and Gijon, Spain. He has presented lectures in Belgium, France and Spain . 

Abstract:

Introduction
A method to achieve complete oral rehabilitation with predictable success.

Applicable to oral rehabilitations with fixed prosthesis on teeth and/or implants. We use the fixed provisional restorations to determine the centric occlusion and dental morphology for an optimal functional outcome on a periodontal, phonetic and aesthetic level.

Materials and methods.
We prepare every case of rehabilitation in a classical way, using die cast models, diagnostic wax up, CBCT scan, surgical guide and a thermoplastic mold of our wax up in order to achieve provisional methacrylate crowns made intra orally.

In order to deprogram the masticatory muscles and finding the centric occlusion a Lucia Jig is then incorporated in the provisional crowns. After a minimal time of ten minutes the centric position is located. Adding methacrylate posterior occlusal stability and lateral guidance is optimized. Esthetic and phonetic adaptations are made.

If there are neither subjective nor objective problems the next weeks of follow up, we scan our provisional bridge. This virtual bridge then will be positioned on the virtual model and all the parameters controlled. Finally the technician makes the reduction on the virtual structure for later ceramic covering and this design is send to the Zirconia milling machine

Results .
Achieving the occlusion in centric relation, reestablishing the TMJ in its physiological position makes us realize full arch rehabilitations with a very good long term prognosis.

Conclusion.
The Tempocopy protocol allows us to work with much more predictability in aspects of occlusion, periodontics, phonetics and aesthetics  

Biography:

Edda Tobiasch, female, molecular biologist, graduated from biology department, Technical University Kaiserslautern. Dr. Tobiasch worked as post-doc on signal transduction pathways at the German Cancer Research Center (DKFZ) in Heidelberg, the Research Center Karlsruhe, and the University of Heidelberg. She was Instructor at Harvard Medical School, Boston, USA and is now professor of the Department of Natural Sciences, Bonn-Rhine-Sieg University of Applied Sciences. She was awarded for "Outstanding Scientific Research" at the DKFZ (1993), as "External Expert (2008) and Internal Expert (2009), Foreign Member of the Ph.D. School" and “visiting professor” (2011) at the University Palermo, Italy. She is a member of the Steering Committee of the Stem Cell Network North Rhine Westphalia

Abstract:

For successful and long-time implant anchorage a sufficient vertical and transversal bone level is necessary. Several cell types are under investigation for reconstruction of the jaw bone for implant anchorage. Next to mesenchymal stem cells from different sources such as adipose tissue, dental follicle cells and bone particles, both of ecto-mesenchymal origin are of major interest for non-vascularized bone autografts. Such bone particles are produced during the implant-bed preparation or are created by bone ablation with surgical round-drills and can be collected with a bone filter integrated into surgical suction pipe. They are thus, as a side-product during surgery available without any additional invasive procedures.

Here we show that ecto-mesenchymal stem cells compared to mesenchymal stem cells are pre-committed towards hard tissues. As ionotrophic P2X and metabotrophic purinergic (P) 2 receptors express a distinct pattern during the development of a tooth in various tissues of the follicle, we investigated the effect of artificial ligands of these receptors on the differentiation process. The administration of the selective P2X7 antagonist A740003 led to an enhanced matrix mineralization, confirming the functional role of P2X7 during late osteogenesis. Interestingly this effect can be focused into collagen scaffold material via drop-on and drop-in only, when based into a petri dish. This mineralization enhancement is correlated to specific chemical scaffold-medium-interactions.

Taken together, the use of ectomesenchymal stem cell together with an antagonist for the P2X7 receptor can improve in vitro osteogenesis. This effect can be further enhanced and localized into a collagen sponge. Such a functionalized scaffold might be applied in regenerative dentistry for optimized osseointegration of dental implants.

Biography:

Yılmaz Umut Aslan has completed his graduate education in Marmara University, Faculty of Dentisty in 2006. He became a Research Assistant in  the Faculty of Dentistry Department of Prosthodontics in the same year. He  became an Associate profesor in 2014 and became a Vice Dean in 2016. He currently works  Vice  Dean and  Teacher of Prosthodontic Departments in Marmara  University, Faculty of Dentistry, Department of Prosthodontics. 

Abstract:

Depending on the developments in computer technologies, implant surgery techniques have several improvements during the last years. With the development of these techniques nowadays, it is expected that edentulous  patients will have both implants and prostheses on the same day. Since it is very important for a patient to have teeth in a day, flapless surgical technique with computer guided procedure is chosen for the immediate loading of implants. Advancements in three-dimensional (3D) imaging technology have helped to us  allowing better visualization of the soft and hard tissues. This improvement facilitates implant treatment planning related to anatomical and prosthetic conditions, leading to a more predictable outcome. Currently, 3D planning software programs are available to transfer the information from a digital 3D pl ning environment to the intraoperative surgical  field by means of computer aided or guided surgery. Using  computer guided protocol, it is a successful treatment option for edentulous patients who demand teeth on the same day . So the best way to obtain this is to use computer and scanning techonologies. Thats how patients has the immediate smile wtih minimum pain.

Computer guided implants can be a successful treatment alternative for immediate loaded implants in edentulous patients. The patients were satisfied with the treatment outcome. This modern approach has many advantages like patient satisfaction, soft tissue management and precision of implant locations. The purpose of this presentation is to demonstrate how to use Computer guided novel approach in treatment of edentulous patients.  

Biography:

Abstract:

Large bone defects require fabricated bone constructs that consist of three main components: an artificial extracellular matrix scaffold, stem cells with the potential to differentiate into osteoblasts, and bioactive substances, such as osteoinductive growth factors to direct the growth and differentiation of cells toward osteogenic lineage within the scaffold. Scaffolds provide a 3D environment for cell seeding and proliferation as well as filling bone defects while affording mechanical competence during the process of bone regeneration. Today, scaffold development is focused on inorganic-organic composites (hybrids), mainly prepared using natural and synthetic polymers (i.e. collagen, polysaccharides), and inorganic hydroxylapatite (HA), tricalcium phosphate (TCP). In recent years, tissue engineers used various modifications such as addition of bioactive molecules or nanoparticles to enhance attachment and proliferation of stem cells on the scaffold. Thus, the application of so-called “smart scaffolds” enhances osteogenic differentiation of stem cells. [1] In particular, purinergic receptors, P2X and P2Y play a key role in osteogenic lineage commitment of human mesenchymal stem cells (MSCs). Via addition of corresponding P2X/Y receptor ligands (agonists, antagonists) the differentiation process can be triggered towards osteoblast formation.

[2]The main focus of this contribution is the correlations between scaffold structures, both bulk andsurface and corresponding cell behavior, i.e. adhesion and differentiation. The human MSCs were gained through isolation of jar bone chip and liposuction material harvested during surgery
intervention. Scaffold structure analysis to investigate scaffold hybrid materials (human, bovine,artificial) provides information on their chemical composition, 3D bulk and surface structure. Thus, FTIR spectroscopy, X-ray diffraction (XRD), small angle X-ray scattering (SAXS), scanning electron microscopy (SEM) and zeta potential measurements will be discussed to explain the hybrid structure-property relationships.Three scaffold materials (collagen, bovine, artificial) were analyzed regarding their chemical composition, 3D bulk and surface structure. Administration of selective P2Y1 antagonists led to an enhanced matrix mineralization thus confirming the functional role of P2X7 during osteogenesis.

Biography:

Yasemin Kulak Ozkan  has completed her graduate education in Marmara University, Faculty of Dentisty in 1987. She became a Research Assistant in  the Faculty of Dentistry Department of Prosthodontics in the same year. She became an Associate profesor in 1996 and became Professor in 2001. She became a Vice Dean in 2004-2007.  She was elected president of TPID (Turkish Prostodontics and Implantology Association) at the 2016 and since 2014 she has  been running the dean of University  of Marmara Faculty of Dentistry  and the Head  of the department of prosthesis,Istanbul, TURKEY. She has various  published more than 60  papers in reputed journals and has been serving as an editorial board member of repute.        

Abstract:

The aims of this clinical study were to compare the marginal and internal adaptation of all-ceramic crowns generated with CAD/CAM before cementation and examine the clinical outcomes at baseline and 6th  month after luting. Materials and methods: A total of 45 crowns were placed in 38 patients, including 15 lithium disilicate ceramic crowns (LDS, IPS e.max CAD Blocks, Ivoclar, Vivadent, Schaan, Liechtenstein), 30 lithium disilicate stengthened lithium aluminosilicate glass-ceramic crowns (LAS, n!ce® CAD Blocks, Institut Straumann AG, Basel, Switzerland). The marginal and internal gaps of crowns were recorded by using a replica technique. The replica specimens were sectioned bucco-lingually and mesio-distally and the thickness of silicone layers was examined by computerized light microscope at × 40 magnification. Twenty reference points per tooth were measured, and mean marginal and internal gaps were recorded. Restorations were clinically assessed at baseline and 6 months after cementation by using modified USPHS criteria, plaque and gingival indexes and patients satisfaction criteria. Data was analysed by using “Mann-Whitney U” and “Wilcoxon Signed Rank” tests (P<0.05). Results: After 6 month-observation period, total survival rates of LDS and LAS groups were 100%. There were no clinically identified cases of crown fracture or surface chipping. The mean marginal gaps were 53. 2963 (± 11. 2691) μm for LDS group and 51. 6703 (± 11. 2381) μm for LAS group. The highest gap value was observed at the occlusal area and lowest one at the marginal area (P<0,05). There was no significant difference in relation to material difference. Conclusions: Regarding all of the clinical evaluation criteria all 45 crowns exhibited clinically acceptable scores within an average evaluation time of 6 months. Early results indicate that LAS crowns may be an effective option for all-ceramic restorations.
 

Biography:

Dirk Neefs  graduated as a dentist in 1987 at Brussels University, specialized in oral rehabilitations with dental implants at Liege University in 1992. He has his dental Office in Antwerp Belgium and Oviedo and Gijon, Spain. He has presented lectures in Belgium, France and Spain . 

Abstract:

Introduction
A method to achieve complete oral rehabilitation with predictable success.

Applicable to oral rehabilitations with fixed prosthesis on teeth and/or implants. We use the fixed provisional restorations to determine the centric occlusion and dental morphology for an optimal functional outcome on a periodontal, phonetic and aesthetic level.

Materials and methods.
We prepare every case of rehabilitation in a classical way, using die cast models, diagnostic wax up, CBCT scan, surgical guide and a thermoplastic mold of our wax up in order to achieve provisional methacrylate crowns made intra orally.

In order to deprogram the masticatory muscles and finding the centric occlusion a Lucia Jig is then incorporated in the provisional crowns. After a minimal time of ten minutes the centric position is located. Adding methacrylate posterior occlusal stability and lateral guidance is optimized. Esthetic and phonetic adaptations are made.

If there are neither subjective nor objective problems the next weeks of follow up, we scan our provisional bridge. This virtual bridge then will be positioned on the virtual model and all the parameters controlled. Finally the technician makes the reduction on the virtual structure for later ceramic covering and this design is send to the Zirconia milling machine

Results .
Achieving the occlusion in centric relation, reestablishing the TMJ in its physiological position makes us realize full arch rehabilitations with a very good long term prognosis.

Conclusion.
The Tempocopy protocol allows us to work with much more predictability in aspects of occlusion, periodontics, phonetics and aesthetics  

Biography:

Edda Tobiasch, female, molecular biologist, graduated from biology department, Technical University Kaiserslautern. Dr. Tobiasch worked as post-doc on signal transduction pathways at the German Cancer Research Center (DKFZ) in Heidelberg, the Research Center Karlsruhe, and the University of Heidelberg. She was Instructor at Harvard Medical School, Boston, USA and is now professor of the Department of Natural Sciences, Bonn-Rhine-Sieg University of Applied Sciences. She was awarded for "Outstanding Scientific Research" at the DKFZ (1993), as "External Expert (2008) and Internal Expert (2009), Foreign Member of the Ph.D. School" and “visiting professor” (2011) at the University Palermo, Italy. She is a member of the Steering Committee of the Stem Cell Network North Rhine Westphalia

Abstract:

For successful and long-time implant anchorage a sufficient vertical and transversal bone level is necessary. Several cell types are under investigation for reconstruction of the jaw bone for implant anchorage. Next to mesenchymal stem cells from different sources such as adipose tissue, dental follicle cells and bone particles, both of ecto-mesenchymal origin are of major interest for non-vascularized bone autografts. Such bone particles are produced during the implant-bed preparation or are created by bone ablation with surgical round-drills and can be collected with a bone filter integrated into surgical suction pipe. They are thus, as a side-product during surgery available without any additional invasive procedures.

Here we show that ecto-mesenchymal stem cells compared to mesenchymal stem cells are pre-committed towards hard tissues. As ionotrophic P2X and metabotrophic purinergic (P) 2 receptors express a distinct pattern during the development of a tooth in various tissues of the follicle, we investigated the effect of artificial ligands of these receptors on the differentiation process. The administration of the selective P2X7 antagonist A740003 led to an enhanced matrix mineralization, confirming the functional role of P2X7 during late osteogenesis. Interestingly this effect can be focused into collagen scaffold material via drop-on and drop-in only, when based into a petri dish. This mineralization enhancement is correlated to specific chemical scaffold-medium-interactions.

Taken together, the use of ectomesenchymal stem cell together with an antagonist for the P2X7 receptor can improve in vitro osteogenesis. This effect can be further enhanced and localized into a collagen sponge. Such a functionalized scaffold might be applied in regenerative dentistry for optimized osseointegration of dental implants.

Biography:

Yılmaz Umut Aslan has completed his graduate education in Marmara University, Faculty of Dentisty in 2006. He became a Research Assistant in  the Faculty of Dentistry Department of Prosthodontics in the same year. He  became an Associate profesor in 2014 and became a Vice Dean in 2016. He currently works  Vice  Dean and  Teacher of Prosthodontic Departments in Marmara  University, Faculty of Dentistry, Department of Prosthodontics. 

Abstract:

Depending on the developments in computer technologies, implant surgery techniques have several improvements during the last years. With the development of these techniques nowadays, it is expected that edentulous  patients will have both implants and prostheses on the same day. Since it is very important for a patient to have teeth in a day, flapless surgical technique with computer guided procedure is chosen for the immediate loading of implants. Advancements in three-dimensional (3D) imaging technology have helped to us  allowing better visualization of the soft and hard tissues. This improvement facilitates implant treatment planning related to anatomical and prosthetic conditions, leading to a more predictable outcome. Currently, 3D planning software programs are available to transfer the information from a digital 3D pl ning environment to the intraoperative surgical  field by means of computer aided or guided surgery. Using  computer guided protocol, it is a successful treatment option for edentulous patients who demand teeth on the same day . So the best way to obtain this is to use computer and scanning techonologies. Thats how patients has the immediate smile wtih minimum pain.

Computer guided implants can be a successful treatment alternative for immediate loaded implants in edentulous patients. The patients were satisfied with the treatment outcome. This modern approach has many advantages like patient satisfaction, soft tissue management and precision of implant locations. The purpose of this presentation is to demonstrate how to use Computer guided novel approach in treatment of edentulous patients.  

Biography:

Abstract:

Large bone defects require fabricated bone constructs that consist of three main components: an artificial extracellular matrix scaffold, stem cells with the potential to differentiate into osteoblasts, and bioactive substances, such as osteoinductive growth factors to direct the growth and differentiation of cells toward osteogenic lineage within the scaffold. Scaffolds provide a 3D environment for cell seeding and proliferation as well as filling bone defects while affording mechanical competence during the process of bone regeneration. Today, scaffold development is focused on inorganic-organic composites (hybrids), mainly prepared using natural and synthetic polymers (i.e. collagen, polysaccharides), and inorganic hydroxylapatite (HA), tricalcium phosphate (TCP). In recent years, tissue engineers used various modifications such as addition of bioactive molecules or nanoparticles to enhance attachment and proliferation of stem cells on the scaffold. Thus, the application of so-called “smart scaffolds” enhances osteogenic differentiation of stem cells. [1] In particular, purinergic receptors, P2X and P2Y play a key role in osteogenic lineage commitment of human mesenchymal stem cells (MSCs). Via addition of corresponding P2X/Y receptor ligands (agonists, antagonists) the differentiation process can be triggered towards osteoblast formation.

[2]The main focus of this contribution is the correlations between scaffold structures, both bulk andsurface and corresponding cell behavior, i.e. adhesion and differentiation. The human MSCs were gained through isolation of jar bone chip and liposuction material harvested during surgery
intervention. Scaffold structure analysis to investigate scaffold hybrid materials (human, bovine,artificial) provides information on their chemical composition, 3D bulk and surface structure. Thus, FTIR spectroscopy, X-ray diffraction (XRD), small angle X-ray scattering (SAXS), scanning electron microscopy (SEM) and zeta potential measurements will be discussed to explain the hybrid structure-property relationships.Three scaffold materials (collagen, bovine, artificial) were analyzed regarding their chemical composition, 3D bulk and surface structure. Administration of selective P2Y1 antagonists led to an enhanced matrix mineralization thus confirming the functional role of P2X7 during osteogenesis.